A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.

نویسندگان

  • Yuuta Fujikawa
  • Fumika Morisaki
  • Asami Ogura
  • Kana Morohashi
  • Sora Enya
  • Ryusuke Niwa
  • Shinji Goto
  • Hirotatsu Kojima
  • Takayoshi Okabe
  • Tetsuo Nagano
  • Hideshi Inoue
چکیده

We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.

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عنوان ژورنال:
  • Chemical communications

دوره 51 57  شماره 

صفحات  -

تاریخ انتشار 2015